IAQ Mold Lab SOP
More Information
InspectAPedia TM Home & Site Map
Air Conditioning
InspectAPedia Bookstore
Electrical
Environment
Exteriors
Heating
Home Inspection
Insulate Ventilate
Interiors
Mold Inspect/Test
Plumbing
Water
Septic
Roofing
Structure
Accuracy & Bias Pledge
Contact Us
American Home Service IAQ Investigation Field Equipment Specifications & SOPs
MoldAPedia ©
- Mold particle count procedures
- Mold concentration count procedures
- Forensic microscopy lab SOPs
This document summarizes currently popular indoor air quality equipment and sampling devices and reviews methodology for their use, laboratory procedures, and proper calculating procedures to produce particle concentrations and exposure assessments for investigating the indoor environment. More in-depth, un-biased, expert information on these topics and on building defect inspection, diagnosis, & repair can be found at More Information below. © Copyright 2008 Daniel Friedman, All Rights Reserved. Information Accuracy & Un-Biased Reporting are Assured for this website - see pledge link at below-left. New York State License # 16000005303 -- Contents.
IAQ Laboratory SOP(Standard Operating Procedure) Indoor Air Monitoring and Testing Equipment - Particle Sampler Trace calculations recap
Related IAQ Investigation Lab & Field SOP's
- Trace Calcs recap.doc - this document inspect-ny.com/sickhouse/IAQEquip.htm © 2007 Daniel Friedman - All Rights Reserved
- For most-recent microscope optical measurements: field width data and calibration see: Olympus-CH-2.xls & Polam Optics.xls
- For most-recent calculations of % of trace represented by n cross-scans see: Particle Counts.xls
- For automated calculations of particles or spores per cubic meter of air (spores/M3) from raw airborne particle counts see: @SporeCountWork.xls
- For spore count worksheets see: sheet 2 of @SporeCountWork.xls
Calibration is repeated no less than when an objective is removed/replaced or other microscope optical or stage components are removed/replaced, or if a new technician is employed.
|
Sampling Device |
Trace length |
Nom. Trace™ Width |
Flow Rate in LPM |
Pump timer |
Calibrated |
|
Sampling Cassettes™ used with IAQ Sampling Pumps™™™ (Microscope slides, adhesive tape, and other collection media are also used) |
|||||
|
Air-O-Cell™ disposable cassette (Zefon) |
14.4mm |
1.1mm |
15 |
2-10 min. typical |
See indiv. Pump device d50=2.6u |
|
Allergenco D™ disposable
cassette ( |
14.0mm nominal |
1.1 mm nominal |
15 lpm recommended, (operates at 5-20 lpm) |
1-10 min. typical |
d50=1.7u |
|
Cyclex-D™ disposable cassette |
Round media |
|
20 lpm |
2-10 min typical |
d50=0.8u |
|
Micro 5™ disposable cassette |
Round media |
|
5 lpm |
5 min. typical |
1" tube if using Gilair-5 pump d50=0.8u |
|
25mm MCE filter cassette |
25mm MCE filter |
Filter area |
Various |
|
0.45u (F25045) |
|
37mm MCE filter cassette |
37mm MCE Filter |
Filter area |
various |
|
0.8u (F37080) |
|
Bi-Air™ disposable cassette |
Dual trace 25mm MCE filter |
Trace area |
3.5 lpm, ™™ |
10 min grab, 2-4-hr. TWA sample |
|
|
Adhesive tape-clear ™" |
Surface particle sampling mold or settled dust/debris Scotch™ Gloss Finish Multi Task Tape 34-8507-5365-7 |
||||
|
Sterile swab |
Culture collection |
||||
|
IAQ Air Sampling Pumps and Particle Collection Equipment |
|||||
|
Allergenco Mk3™ time lapse slit-impaction sampler |
14.5mm, up to 12 traces/slide |
1.1mm |
15 nom. lpm |
1min to 24-hrs™ multi-trace |
5/17/01 MK2 d50=0.67 |
|
Burkard PAS#1 |
14.0mm |
2.0mm |
10 lpm |
0-9 min |
Factory d50=2.52u |
|
Burkard PAS#2 |
14.0mm |
2.0mm |
10 lpm |
0-9min |
Factory |
|
Intermatic (used with EMS Megalite below) |
NA |
NA |
NA |
1 second to |
Factory |
|
|
NA |
NA |
3-30 lpm |
Separate duplex timed receptacle |
Factory, pump-mounted rotameter, Lab bubble tube 10/10/05 0 lab |
|
Gil-Air-5-RC pump |
NA |
NA |
1-5 adjustable |
1 min to 8-hrs |
1/15/04 Lab, Zefon bubble tube (ZBT) + (OD) in field at each use |
|
RadioShack Pump |
NA |
NA |
10 lpm |
Manual on-off |
Lab 10/10/2003™ -9/5/2005 (ZBT) in lab & (ZR) in field at each use |
|
Notes: particle collection efficiency d50 for other common devices: (J. Tucker) AGI-30 liquid impinger ™ 0.31; SAS Slit-to-Agar™0.67; Cyclex impactor 1.80; RCS radial impactor 4.00, Andersen N6 hole impactor 6.61, Rotorod 10.0. |
|||||
|
Gas Detection Equipment |
|||||
|
Sensidyne Gastec pump (gas detection) |
NA |
NA |
50 or 100 cc/ ™ /full stroke |
Manual stroke, manual count indicator |
Leak test 11/16/04 8/5/2005 |
|
Draeger accuro pump (gas detection) ARUK-FO13 #1-0893171516K09 exp F013 Serial 6400000 |
NA |
NA |
100 cc/stroke |
Manual, stroke completion indicator, automatic stroke counter |
Leak test 11/16/2004 9/5/2005 |
|
RTCA Pump#1 Rotary vane vacuum |
NA |
NA |
14.28 |
11.27 min |
Lab 6/11/04™ total 160.94L |
|
RTCA Pump#2 |
NA |
NA |
Tbd |
Tbd |
Tbd |
|
Other Equipment & Specifications |
|||||
|
Tramex |
electronic moisture encounter |
||||
|
Delmhorst |
twin-point electronic resistance moisture meter |
||||
|
Exergen ™ |
Infrared scanner ™ surface temperature |
||||
|
OD = flow indicator on device (subject to lab calibration) ZR = Zefon rotameter, for field use, verified using ZBT in lab ZBT = Zefon bubble tube (precise), multiple timed events to measure actual LPM Factory = original calibration provided by factory or independent consultant to factory. |
|||||
This document supports our General Lab Procedures and Particle
Trace Count Procedures.
See www.inspect-ny.com/sickhouse/TraceCalcs.htm
More Information on Indoor Air Quality and Toxic Mold Testing & Building Diagnostic Inspections and Repairs
InspectAPedia TM Home & Site Map - Building Inspection, Diagnosis, & Repair, Environmental Inspection & Testing - Research Website
The Mold Information Center: What to Do About Mold in Buildings, When and How
to Inspect for Mold, Clean Up Mold, or Avoid Mold Problems
Home Inspection Construction Consulting Services & advice for home buyers
Use this simple, economical mold test kit by following
our instructions on how to collect and mail mold samples to our lab
Environmental
Inspection, Testing, & Diagnosis On-Site IAQ, Gas, Air Testing, Mold Investigation, Sick Building Diagnosis,
Lab Services, & Remediation Plan Preparation - indoor air quality testing, problem source determination, supporting lab work, written remediation plan addressing
removal of environmental and other hazards and prevention of their recurrence.
Contact Daniel Friedman for website content suggestions or for fee-paid consulting