Contents: Slide Preparation
Slide Preparation Procedure
Slide Stain & Media Recipes
Permanent Slide Mounts
Gelvatol
Meltmount
Mowiol
The Stains File
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Microscope slide preparation methods - mold pollen bioaerosols - for indoor air quality analysis - Mold Testing Laboratory SOP MoldAPedia ©
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- Microscope slide preparation methods - mold pollen bioaerosols - for indoor air quality analysis - Mold Testing Laboratory Standard Operating Procedures SOPs
- Recipes and Formulas for microscope slide preparation mounting media
- Names and Sources for microscope slide mount preparation chemicals, media, and equipment, especially for aerobiologists and forensic particle examination
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This is a collection of aerobiology and mold testing lab standard operating procedures - SOP's and slide preparation recipes
useful for identification of mold, pollen, animal dander, skin cells, mite fecals, mouse dander, and other airborne allergens and bioaerosols.
Comments and corrections are
requested.
© Copyright 2008 Daniel Friedman, All Rights Reserved. Information Accuracy & Bias Pledge is at below-left. Use links at the left of each page to navigate this document or to view other topics at this website. Green links show where you are in our document or website.
Slide Preparation Procedures & Guidelines
- Lab procedures: Cargille meltmountTM slides for rapid field or laboratory use, how to prepare
- Lab procedures: Microscopy - Microscope techniques and laboratory procedures for indoor air quality, toxic mold identification, & bioaerosol investigations
- Lab procedures: Slide preparation - tips on mold lab procedures
Microscope Slide Stain and Media Recipes
How to Prepare Permanent Microscope Slide Mounts
Here is a list of some candidates. WARNING: some of these involve dangerous chemicals, possibly carcinogenic (cancer-causing) and should
be used only with expert advice..
- Canada Balsam - traditional, excellent slide mounting media, generally not available. See Cargille's "Meltmount" alternative.
- Clear nail polish - experimental -DF
- Damar Varnish - see Brinkworth and Smith article below
- Fructose Larry Legg's recipe
- Glycerin or glycerine jelly. DF note: we've been disappointed that even after adding phenol or other preservatives some of our glycerine jelly slides become cloudy or even opaque with age. We
no longer recommend this mountant for permanent slide mounts.
- Gum Arabic (Acacia) or similar water soluble gums
- Hyrax / Dirax high refractive index mountants - Northern Biological Supplies
- Lactic acid combined with phenol (carbolic acid), polyvinyl alcohol, glycerine, water. (Not for caliciferous materials - you'll find that materials like drywall dust, gypsum, some plaster contents, will dissolve and then recrystalize in this media)
- Loctite glass bond - local auto supply stores, needs UV light to cure - experimental, Brinkworth
and Smith [DF note: I've had good results using this slide preparation media for permanent mounts of certain
particles; we need to research the refractive index but in general that's not been a problem with paint samples nor
with fungal spores or other common indoor building dust particles. We use a halogen lamp to generate the UV needed to cure the mountant.
- Meltmount - see below
- Mowiol - available from Burkard as a replacement for Gelvatol - data to be added below
- NBS Bioseal Two
- Numount - from Brunel microscopes & Northern Biological Supplies
- Turtox - no longer available, formula sought. see Howey's article
- Water soluble gums - gum Arabic (Acacia)
- Some microscope slide mounting technique articles
How to Use Gelvatol to Prepare Microscope Slides - A Detailed Step by Step Procedure
Gelvatol may be used as a permanent slide mounting medium for mold spore or pollen slides.
Gelvatol has been replaced by Mowiol from Burkard - see Mowiol below.
- 100 ml H2O (I would use distilled water)
- 35g Gelvatol (or Mowiol from Burkard)
- Polyvinyl alcohol (from Fisher Scientific, or other sources)
Heat to dissolve, not to much. Then add:
- 50 ml Glycerine (Glycerol, Fisher Cat. No. ?)
- 2g Phenol (as a preservative if needed)(Phenol smells a lot and it is no good for health, it gets yellow with time, I do not use it)
Add
- ACID fuchsin (as a colorant for SPORES if needed - Fisher Cat. No. AC22790-0250) Just use a few crystals, mix and wait. Add more if wanted, do not use too much as it will hide the structures.
OR BASIC fuchsin (as a colorant for POLLEN if needed - Fisher Cat. No. NC9653986)
OR no colorant
Keep closed tight because it gets harder as it gets in contact with air.
Source: Burkard company; I was told that they call the "polyvinyl alcohol" Gelvatol, available from Burkard.
Meltmount for Microscopic Slide Preparations
This product, available from Cargille Laboratories, is a contemporary, non-toxic replacement for Canada Balsam and has the same refractive index. Instructions
for preparing slides using MeltMount are available from Cargille. I use this product in the lab for permanent slide mounts.
Mowiol
This replacement for Gelvatol is provided by Burkard. I'll add more information here when I can
find it.
The Stains File http://stainsfile.info/StainsFile/jindex.html
This is the most extensive list I've found of slide preparation stains. However it's quite shy on stains for pollen and mold
spores. Above are listed stains and recipes of particular interest to aerobiologists and for which I have detail or alternatives beyond the information at the Stains File.
More Information on Building Diagnostic Inspections and Repairs
- Lab procedures: Airborne Particle Trace Calculations Recap - how to calculate airborne particle concentrations for mold, IAQ, allergens, or other forensic particle studies - A Laboratory SOP
- Lab procedures: Calibration and Mold Test Laboratory SOP documents?
- Lab procedures: Cargille meltmountTM slides for rapid field or laboratory use, how to prepare
- Lab procedures: Microscopy - Microscope techniques and laboratory procedures for indoor air quality, toxic mold identification, & bioaerosol investigations
- Lab procedures: Slide preparation - tips on mold lab procedures?
Fisher Scientific lab supplies
ICN Biomedicals biomedical products, books, supplies and MSDS information
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